Crossflow microfiltration of recombinant Escherichia coli lysates after high pressure homogenization.

Cloning and optimization of phytase enzyme gene expression in Escherichia coli

Introduction Phytase is an enzyme that has the ability to break down phytic acid into myoinositol and mineral phosphate, and widely uses as an additive in animal foods. The aim of this study was to achieve a high level of bacterial phytase expression in PET26b expression host. Materials and Methods To generate the recombinant phytase enzyme, the target gene was introduced into the expression ...

Experimental Study of Membrane Fouling during Crossflow Microfiltration of Yeast and Bacteria Suspensions: Towards an Analysis at the Microscopic Level

Microfiltration of model cell suspensions combining macroscopic and microscopic approaches was studied in order to better understand microbial membrane fouling mechanisms. The respective impact of Saccharomyces cerevisiae yeast and Escherichia coli bacteria on crossflow microfiltration performances was investigated using a multichannel ceramic 0.2 µm membrane. Pure yeast suspensions (5 µm ovoid...

Optimization of secretory expression of recombinant hGM-CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method

Human granulocyte macrophage colony stimulating factor (hGM-CSF) has many therapeutic applications.In this study, in order to verify the purification process, the effect of carbon source, IPTG concentration andpost-induction time on the secretion of recombinant hGM-CSF into the culture medium by recombinantEscherichia coli during high cell density cultivation were evaluated by...

media and specific growth rate selection for high-cell-density cultivation of recombinant escherichia coli producing hGM-CSF in fed-batch process

Single Step Clarification & Capture of a Recombinant Protein from E. coli Osmotic Shock by Crossflow Chromatography

Natrix chromatography membranes combine all the advantages of chromatographic resins (specificity, selectivity, high binding capacities) and conventional membranes (high throughput, ease of operation, scalability) in a single use format. Since Natrix membranes are inherently hydrophilic they are naturally resistant to fouling, and the Crossflow device formats permit simultaneous Clarification a...